Sentence examples for blocking solution was applied from inspiring English sources

The primary antibody diluted in blocking solution was applied for one hour, except E-cadherin which was stained overnight at 4°C.

After transfer and blocking with 5% skimmed milk in TBS, the primary antibody diluted in blocking solution was applied for 2 hours at 37°C.

After three washes in PBS (5 min each), the secondary antibody, diluted in blocking solution, was applied for 1 h (Alexa 488, goat anti rabbit, 1∶1000, Invitrogen, Karlsruhe, Germany).

Anti-cyclin D1 antibody (1/500 in blocking solution) was applied overnight at 4°C.

Anti-MyoVI antibody in blocking solution was applied overnight at 4°C.

After cutting, 14 μL of blocking solution was applied to the first inlet to minimize nonspecific protein adsorption during storage.

Secondary antibodies in blocking solution were applied for 1 h at room temperature (RT).

Antibodies (rabbit anti-MDGA2A 1:300, mouse anti-GFP (Roche) 1 200 in blocking solution) were applied and incubated ON at 4°C.

HRP-conjugated secondary antibodies (Jackson Research Laboratories , Inc in blocking solution were applied to the membrane after four TBST washes and incubated for 2 h at room temperature.

Then, rhodamine-conjugated anti-rabbit IgG and FITC-conjugated anti-mouse IgG polyclonal antibodies (each 1 500 in blocking solution) were applied for 30 min, and the slides were observed using a fluorescence microscope (Leica Microsystems).

After washing, the respective secondary antibodies (goat anti-rat 488, goat anti-mouse 568; goat anti-mouse 488, goat anti-rabbit 568, all Molecular Probes (Eugene, OR, USA), 1/1000 in blocking solution) were applied for 2 h at room temperature.