Sentence examples for blocking solution was added from inspiring English sources

An additional 90 µl of blocking solution was added to the first well of the dilution series.

After initial blocking, the volume was discarded and 100 µl of blocking solution was added to each well.

Biotin blocking solution was added, samples were incubated and centrifuged.

After three PBS washes, secondary antibody in blocking solution was added for 3 hr.

Goat serum blocking solution was added drop-wise and slices were incubated for 5 h.

Then serially diluted serum in blocking solution was added in duplicate and incubated 1 h at 37°C.

Primary antibodies diluted in blocking solution were added and incubated for ∼1 h at room temperature.

For IgG1 binding assays, anti-HA IgG1 in blocking solution were added to wells and incubated for 1 hour at room temperature, after which bound IgG1 was detected using a peroxidase-conjugated mouse anti-human IgG antibody (Jackson).

Cells on glass coverslips were fixed in 100% methanol for 5 min at −20°C, washed in PBS and incubated in blocking solution (1% FBS/PBS) for 30 min. Primary antibodies in blocking solution were added for 2 h at room temperature (RT): 1∶1000 rabbit α-GFP (Ab290, Abcam) or 1∶500 mouse α-γ-tubulin (GTU-88, Sigma).

200 μl TdT enzyme/reaction buffer, anti-digoxigenin antibody/blocking solution and blocking solution were added per cell-based microarray and 50 μl per 6-well plate coverslip.

After washing three times with TBST, secondary antibodies diluted in blocking solution were added and incubated for 1 hour at room temperature.