Sentence examples for blocking solution to a from inspiring English sources

Secondary antibody was diluted in fresh TBST and 1% blocking solution to a final concentration of 1∶100 000, and incubated for one hour at room temperature under constant agitation.

Arrays were prepared for detection by blocking in 2× PBS, 0.1% Tween-20, 1% Acetylated BSA for 15 min at RT. Streptavidin-Alexa Fluor 647 (Molecular Probes, Eugene, OR) was diluted in blocking solution to a final concentration of 1 µg/mL, and arrays were incubated with this solution for 30 min at RT while protected from light.

D5F10 and the isotype control were diluted in Emerald antibody diluent (Cell Marque, Rocklin, CA) to a concentration of 4 μg/ml for IHC and were diluted in 2 % bovine serum albumin (BSA, Calbiochem, Billerica, MA) in Tris-buffered saline with 0.05 % Tween (TBST) blocking solution to a concentration of 0.2 μg/ml for Western blots.

A monoclonal anti-desmosomal protein antibody (Sigma, St Louis, MO) was added to the blocking solution at a 1∶400 dilution and the cells were incubated at 37°C for 2 h.

A mouse monoclonal anti-human cystatin C primary antibody (Santa Cruz Biotechnology, Santa Cruz, CA) was added to the blocking solution at a 1 1000 dilution and incubated overnight at 4°C.

The tissue sections were subjected to antigen retrieval by microwaving in 10 mM citrate buffer for 30 min. The sections were incubated with serum blocking solution (Reagent A) for 10 minutes to block nonspecific binding and then with the primary antibodies in moist chamber for 60 minutes.

Gelatin solution (2% wt/vol) was used as a blocking solution prior to immunoblotting with the antiphosphotyrosine (4G10) antibody.

All primary and secondary antibodies were diluted in blocking solution according to manufacturer's instructions.

Note that sections were incubated in blocking solution without a primary antibody for the negative control.

After incubation in blocking solution, embryos were transferred to a solution with 3G8 and/or 4A6 monoclonal antibody [42] followed by incubation with anti-mouse alkaline phosphatase IgG (Sigma) and developed using SIGMAFAST fast red tablets (Sigma).

Membranes were probed with antibodies in the blocking solution for an additional hour.