Sentence examples for blocking solution made of from inspiring English sources

Afterwards the membranes were incubated at 4°C in blocking solution made of 6% dried non-fat milk in TBS-T buffer (20 mM Tris-HCl, 137 mM NaCl and 0.05% Tween 20, pH 7.6).

After blocking endogenous peroxidase with methanol containing 0.3% hydrogen peroxide (H2O2) for 5 min, sections were incubated with a blocking solution made of 0.4% casein in trisbuffered saline (TBS) to reduce nonspecific binding.

Following the transfer of proteins, the membranes were blocked in 5 % blocking solution made from, non-fat dry milk dissolved in 1x TBST (50 mM Tris.HCl, pH 7.4, 150 mM NaCl + 0.1 % Tween 20) for 1 h.

Sections were then washed in PBS and incubated at room temperature, inside a moist chamber, for 30 min with a blocking solution, which was made of 5% normal goat serum diluted in PBS.

To achieve this, the oocytes/embryos were transferred into drops (made up of a blocking solution, 10 μL of Hoechst 33342 and glycerol) on a slide and covered with a cover slip.

In other words, many of these preferences of the wealthy will reinforce inequality/immobility-inducing policy and block solutions, making this important public opinion research worth keeping a close eye on.

Four aptamer spots were prepared in a small area of 1×4.8 mm2 with five reference spots made of blocking solution.

The blot was incubated overnight with 6H4 monoclonal antibody (Prionics AG) made up in blocking solution at a concentration of 1 30,000.

Goat anti-mouse secondary antibody was diluted at 1 2000 in freshly made blocking solution.

The sections were further incubated in a blocking solution, which consisted of avidin (Cat. no.

After discarding the blocking solution, one ml of blocking solution containing about 10 phage particle was added to the cells and incubated for 1 h at 37°C.