Sentence examples for blocking solution in the from inspiring English sources

Primary antibodies were diluted in blocking solution in the following manner: rabbit anti-NFκB p65 (A) (sc-109 Santa Cruz) 1∶100; mouse anti-β-actin (C4) sc-477788 Santa Cruz) 1∶1000; rabbit PDGFb (H-55) (sc-7878 Santa Cruz) 1∶100; rabbit Histone H3 (#9715 Cell Signaling Technology) 1∶1000 and incubated on the membranes with rocking overnight at 4°C.

Primary antibody was replaced with blocking solution in the negative controls.

After incubation for 1 h at 37 °C with the blocking solution in a preheated humidity chamber, cell cultures were incubated overnight with these PLA probe-linked antibodies (final concentration of 65 μg/ml) at 4 °C.

After incubation for 1 h at 37 °C with the blocking solution in a preheated humidity chamber, tissue sections were incubated overnight with these PLA probe-linked antibodies (final concentration of 65 μg/ml) at 4 °C.

Blocked embryos were incubated overnight with anti-RNA polymerase II CTD pSer5 (1:1000, Abcam Cat# ab5131), washed in PBTw, reblocked in blocking solution and incubated in the secondary antibody (Alexa Fluor 594 goat anti-rabbit IgG(H+L) (1 300, Molecular Probes Cat# a11012) before imaging.

Plates were incubated for 1 h at room temperature with the G12 moAb (1∶1,000 in the blocking solution), A1 moAb (1∶500 in the blocking solution) or 6B5L1 moAb (1∶1,000 in the blocking solution).

Tissues were then washed in PBT and incubated in blocking solution containing the appropriate primary antibody.

Following their incubation in blocking solution, the slides were incubated with the CM2B4 antibody diluted 1 : 100.

Sections were washed three times in PBS (10 min each), incubated in blocking solution (0.5 % Triton X-100, 4%% horse serum, in PBS) for 1 h, and incubated overnight in blocking solution containing the primary antibody.

In brief, following air drying, sections were blocked in 2 % BSA PBS for 15 min before being incubated in blocking solution containing the appropriate primary antibody for 16 h.

Membranes were blocked overnight and then incubated with primary antibody in blocking solution at the appropriate dilution (ZIP4 antiserum 1∶800; ZIP1 antiserum 1∶800) for 2 hr at room temperature.