Sentence examples for blocking solution consisting of from inspiring English sources

Samples containing 80 μg of protein were loaded into 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), proteins were then electrotransferred to a polyvinylidene fluoride (PVDF) membrane (Amersham Bio-sciences, Freiburg, Germany) and blocked at room temperature in a blocking solution consisting of TBST (Tris-buffer saline containing 0.05% Tween-20).

After fixation, blocking solution consisting of 5% Goat Serum (Sigma-Aldrich, UK) in PBST was applied and allowed to block for 10 minutes.

Tissue sections were exposed to a blocking solution consisting of 1X SuperBlock Reagent and 5% Normal Goat Serum in PBS for 1 hour.

Ultrathin sections (70 90 nm) from Unicryl-embedded blocks were incubated for 45 min on pioloform-coated nickel grids with drops of blocking solution consisting of 2% albumin in 0.05 M TBS, 0.9% NaCl, and 0.03% Triton X-100.

Frozen tissue sections were rinsed in 0.5M KPBS and incubated in blocking solution consisting of KPBS containing 1% bovine serum albumin (Fisher, Pittsburgh, PA), 0.4% Triton-X 100 (Sigma), and 1.5% normal donkey serum (Invitrogen) for 2 hours.

The membrane was incubated with a blocking solution consisting of 5% nonfat dry milk in TBST (150 mM NaCl, 50 mM Tris, 0.1% Tween-20, pH 7.5) at room temperature (RT) for 1 h.

This blocking solution consisted of 0.125% poly vinylpyrrolidone) + 0.125% bovine serum albumin + 2.5% sucrose + 7.5 mM sodium azide + 0.1% Tween-20 in water.

Blocking solution consisted of 5% nonfat dry milk in Tris Buffered Saline (TBS) buffer (pH of 7.4 and 0.01 M Tris Base, 153 mM NaCl).

Specimens were then incubated with 3%H2O22 in methanol for 12 min at room temperature to block endogenous peroxidase, washed with PBS (pH 7.5) and incubated for 20 min at room temperature in a protein-blocking solution consisting of PBS supplemented with 1% normal goat serum and 5% normal horse serum.

Differentiated and undifferentiated CL6 cells were fixed with 4% paraformaldehyde (Wako) for 5 min at 4°C and treated with 0.1% triton X-100 (Sigma) in PBS for 20 min at room temperature, then incubated for 20 min at room temperature in a protein-blocking solution consisting of PBS supplemented with 5% normal goat serum (DakoCytomation).

Sections were washed three times for 3 min each with PBS (pH 7.5) and incubated for 20 min at room temperature in a protein-blocking solution consisting of PBS supplemented with 1% normal goat serum and 5% normal horse serum.