Sentence examples for blocking solution composed of from inspiring English sources

The membrane was then incubated at room temperature in a blocking solution composed of 5% skim milk powder dissolved in 1× TBS (10 mM Tris, pH 8.0, and 140 mM NaCl) for 1 h.

After washing with PBS again, the cells were incubated at room temperature for 1 h in a blocking solution composed of PBS containing 2%% non-fat milk.

Briefly, 1 μg of membranes was added to each well for 1 h followed by blocking with 25 μL of blocking solution composed of 3% Bovine serum albumin (BSA) and 1% skim milk powder.

After being permeabilized with 0.1% Triton X-100 in PBS at room temperature for 20 minutes, monolayers were then incubated in blocking solution composed of bovine serum albumin and normal donkey serum in PBS for 1 hour.

After electrophoresis, the proteins were electrotransferred to a nitrocellulose membrane (Hybond ECL; Amersham Biosciences, Buckinghamshire, UK) and incubated with a blocking solution composed of 5% fat dry milk in Tween-containing Tris-buffered saline (pH 8.0, 10 mm Tris, 150 mm NaCl, 0.1% Tween).

The membrane was then incubated at room temperature in a blocking solution composed of 5% skimmed milk powder dissolved in Tris-buffered saline (TBS), pH 7.4, containing 0.1%Tween-20Tween-20h, followed by incubation in the blocking solution with anti-human JNK, p-JNK, p38, p-p38, ATF2, p-ATF2 antibodies separately at 4°C overnight.

For immunolabelling on RPE flat mounts, the blocking solution was composed of PBS with 10% NGS and 1% Triton X-100.

Then, the brains were blocked in a solution of 0.1% NaBH4 in PBS and in a second solution composed of 5% BSA, 0.1% CWFSkin gelatin and 1% NGS (Aurion).

At the incubation site, 1 mL cell suspensions were injected into 1 mL of -70°C cold quenching solution composed of 70% methanol in water using a thermo block above dry ice.

Briefly, the samples were immerged into a solution composed of 2 4% formaldehyde solution, pH 7.4, supplemented with 5% sodium carbonate.

Blocking solution was composed of 2% (v/v) normal goat serum (Gibco, Bornem, Belgium), 0.2% (w/v) powdered milk (Bio-Rad, Hercules, CA, USA), 1% (w/v) bovine serum albumin (BSA, Sigma-Aldrich, St . Louis MO, USA), 0.1M glycine (Bio-Rad), 0.01% Triton X-100 (Bio-Rad), in PBS (Calbiochem, Darmstad, Germany) with 0.2% sodium azide (Sigma-Aldrich,).