Sentence examples for blocking solution at a from inspiring English sources

A monoclonal anti-desmosomal protein antibody (Sigma, St Louis, MO) was added to the blocking solution at a 1∶400 dilution and the cells were incubated at 37°C for 2 h.

Slides were incubated for 1 hr at room temperature, washed three times with PBS and incubated with secondary fluorescent Alex-568 goat anti-human antibody in blocking solution at a concentration of 40 µg/ml.

Sections are then incubated with primary antibody (cG250, a murine Fv grafted human IgG1K - kindly supplied by Ludwig Institute of Cancer Research) applied in blocking solution at a concentration of 25 µg/ml.

The secondary antibody (HRP-conjugated goat anti-human IgM/IgG) was prepared in blocking solution at a concentration of 1 5000 and incubated for 1 hour.

After washing with TBS-T2, secondary antibodies were added in blocking solution at a 1 1000 dilution and incubated at room temperature for 2 h.

The blot was incubated overnight with 6H4 monoclonal antibody (Prionics AG) made up in blocking solution at a concentration of 1 30,000.

Sections were incubated with blocking solution at 37°C in a humid chamber for 1 h.

Cells were incubated with a blocking solution at 37°C for 30 min, and then washed with Wash Buffer A for 5 min twice.

Sections were incubated in blocking solution at 4°C overnight with a combination of mouse anti-α-syn antibody (dilution 1∶250; BD Bioscience) and rabbit anti-CHIP antibody (dilution 1∶200; Calbiochem), or with a combination of mouse anti-BAG5 antibody (dilution 1∶250; Santa Cruz) [11] and rabbit anti-CHIP antibody (dilution 1∶200; Calbiochem).

Subsequently they were incubated overnight with the primary antibodies diluted in blocking solution at room temperature, on a rocking plate.

Methanol-fixed Jurkat cells were incubated with a blocking solution at 37 °C for 45 min, then washed with HBSS for 5 min twice.