Sentence examples for blocking solution and the from inspiring English sources

Briefly, the membranes were blocked for 30 min; 20 µl of anti-digoxigenin-AP Fab-fragments (15 U/20 ml) (Roche) were added to the blocking solution; and the membranes were incubated for 30 min at room temperature.

Endogenous peroxidase activity was inactivated by incubation in blocking solution and the slides then incubated with primary antibody (30 minutes).

Primary antibodies to be tested were added (singly, diluted in 20 μl of blocking solution), and the resulting mixtures were each covered by a cover slip and incubated overnight at 4 °C.

Horseradish peroxidase (HRP -conjugated secondary antibodies were applied for 2 HRP -conjugatedemperature in the blocking secondaryantibodiesmbranes visualized by reaction were the Western Lightning Chemiluminescence ReappliedPerkin Elmer).

The wells were washed for 4 times with blocking solution and the bound phage was eluted with 400 ml of 0.1 M glycine, pH 2.2, neutralized and assayed by using GFP ELISA kit (cat. AKR-121, Cell Biolabs).

Different parameters, such as the amount of immunoreagents, type of the materials, composition of the blocking solution and of the detector reagent mixture, were investigated to provide the optimum assay performance.

In control experiments for possible nonspecific binding of the secondary antiserum, we omitted the primary antiserum, replaced it with blocking solution, and followed the labeling protocol as above.

Sections were incubated overnight at 4°C with gentle agitation in primary antibodies diluted in blocking solution, and washed the following morning.

The appropriate antibodies were diluted in the blocking solution and applied on the cells for 1 h at room temperature; the excess of antibodies was removed by washing with the blocking solution before the 1 h incubation with the corresponding secondary antibodies.

Anti-mitochondrion specific heat shock protein 70 (mtHSP70) antibody (clone JG1, Abcam, www.abcam.com) and anti-á-tubulin (clone YOL 1/34, Abcam) antibody were diluted 1 250 in the blocking solution and applied to the specimens on ice overnight.

Primary and secondary antibodies were diluted in the blocking solution and incubated with the sample overnight with gentle agitation at 4°C.