Sentence examples for blocking serum was applied from inspiring English sources

A total of 10% blocking serum was applied for 20 min at room temperature before application of the primary antibody overnight at 4°C.

Blocking serum was applied, according to the manufacturer's instructions (Vectastain Universal Elite ABC kit, Vector Laboratories, Burlingame, CA, USA).

Blocking serum was applied for 15 min and followed by incubation with rabbit anti-human c-erbB-2 oncoprotein (code No. A 0485, Dako) diluted 1 350.

Blocking serum was applied for 15 min and followed by incubation with rabbit anti-human c-erbB-2 oncoprotein (Dako, Glostrup, Denmark) diluted 1 : 500.

Blocking serum was applied for 20 min and sections were then sequentially incubated in primary mouse anti-human Ki-67 antibody (1 100, clone Ki-S5; Dako, Carpinteria, CA, USA) for 1 hour, biotinylated rabbit anti-mouse IgG (Dako LSAB kit) for 12 min, and ABC reagent (avidin and biotinylated horseradish peroxidase complex) for 30 min (ABC kit; Vector, Burlingame, CA, USA).

Following a further wash in TBSTX, a non-immune blocking solution of normal goat serum was applied for 20 minutes prior to application of cannabinoid receptor 1 antibody (rabbit polyclonal, Abcam, Cambridge, UK) diluted 1∶300 in normal goat serum.

After a rinse in PBS, bovine serum was applied to block non-specific interactions.

After rinsing with Tris-buffered saline, normal horse serum was applied for 30 minutes to block nonspecific antibody binding.

After rinsing with Tris-buffered saline, normal horse serum was applied for 30 minutes to block non-specific antibody binding.

After being rinsed in phosphate buffered saline (PBS), 10% goat serum was applied for 1 h at room temperature to block any nonspecific reactions.

Not all serums are applied with the same frequency.