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The RCA reaction can be launched once the blocking sequence is cut off under DNA break.
As contrasted with conventional mono-functional primer, our primer has three functional domains: a reserved sequence at the 5′-terminal to hybridize with the circular probe, a double-stranded domain to be exposed to DNA break inducements, and a blocking sequence at the 3′-terminal to block the RCA reaction.
Thus, the blocking sequence in the Evx2-Hoxd13 system can be divided into two units—a constitutive blocker and a blocker regulator.
Importantly, the fluorescence of Sp-I recovered to about 90% of that of Sp in the presence of 3-fold molar excess of a DNA strand complementary to the internal blocking sequence and its neighboring bases (Anti-I-DNA), demonstrating that Sp-I can be activated by sequestering the blocking sequence by hybridization.
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To demonstrate the application of different knot blocking sequences in laparoscopic surgery.
An optimality criterion is introduced for sequencing blocks within a given design and tables of optimal blocking sequences are provided for most of the designs given in Sun et al. (Technometrics 39 (1997) 298).
Two isolated antibodies from vaccine recipients, CH58 and CH59, bind to lysine 169 in gp120 V2 (Liao et al., 2013), a position implicated by sieve analysis in blocking sequence-matched HIV-1 strains.
Patients were randomised using random blocking sequences set up before commencing of the study.
Data suggest that CTCF binding to enhancer blocking sequences within the DMR is modulated by methylation status, which results in differential interference of enhancer-promoter communication.
Two fixed block sequences were used: block sequence 1: M S M M C M M S M M C M block sequence 2: M C M M S M M C M M S M Block sequence 1 was administered to participants who performed the S block first during the practice session.
No significant preference in Kd value is observed if the 5′ or 3′ end of the aptamer is partially blocked (sequence i vs sequence iii).
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