Exact(35)
There was smoke and thick dust everywhere, fallen masonry and fittings were blocking sections of the steps and splinters of glass covered the staircase where Picasso's sand-blasted lines hung undamaged.
"There were already protesters lining up and blocking sections of the freeway in LA.
After blocking, sections were incubated with rabbit anti-nanog (Bethyl, Montgomery, USA) in blocking solution overnight at 4°C.
After normal serum blocking, sections were incubated with the primary antibody overnight at 4°C, followed by incubation in biotinylated secondary antibody.
Following post hybridization washes and blocking, sections were incubated for 30 minutes in 1∶100 anti-DIG antibody conjugated with horseradish peroxidase (Roche, Germany) and signal was visualized using tyramide signal amplification (PerkinElmer, Germany).
Endogenous peroxidase was inhibited and after blocking sections in TBS with 0.5% Triton X-100 and 10% goat serum, incubation was performed overnight with rabbit polyclonal anti Ki67 antibody (Ki67P Novocastra, UK).
Similar(25)
That means removing culverts, reconnecting blocked sections of rivers and reclaiming vegetation.
Antibody 4E7(1:1000) were applied to the blocked sections and incubated overnight at 4°C.
The blocked sections were incubated overnight at 4°C with primary antibody (1% BSA in PBST).
Blocked sections were incubated with anti-HMGB1 polyclonal antibody (1 1000 dilution).
The blocked sections were then washed in methanol, running water and three 5 min washes in TBS (pH 7.6).
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