Sentence examples for blocking reagent was added from inspiring English sources

Unbound antibody was removed by washing the plate and a blocking reagent was added.

The primary antibody (anti-PHLPP1 and anti-PHLPP2 from Abcam) in the blocking reagent was added and incubated overnight at 4 °C.

After 20 min at 4°C, the cells were washed, fixed and permeabilised and resuspended in 200 μL PBS/5% FBS; 20 μL FcR blocking reagent was added to the cells for 5 min. Then 0.2 μg FITC-conjugated anti-arginase I or mouse FITC-conjugated IgG1 was added to the cells, and 20 min later the cells were washed.

In situ hybridization was performed as described previously (Schinko et al., 2009) with minor changes: Roche Blocking Reagent was added to the HybeA buffer and used as the standard blocking buffer in all following steps.

Different combinations of blocking reagents were added to the hybridization mixture: Mouse COT-1 DNA (Life Technologies) was added to all mixtures (0.1 μg/ μg RNA) in order to block hybridization of repetitive elements.

Mouse anti‐GFP (1 200) and rat anti‐PECAM1 (1 200) antibodies in Blocking Reagent were added and incubated for 1 h at 37°C.

The blocking reagent was removed and rabbit anti-HCC (diluted 1 1000 in blocking buffer) was added and incubated for 3 h at 37°C.

The blocking reagent was removed and the cells were washed three times in PBS before the PAb was added.

The blocking reagent was removed and 1 1000 PAb or control rabbit IgG in PBST containing 5% skim milk was added for 30 min.

Peroxidase blocking reagent was from Dako.

Western blocking reagent was obtained from Roche Diagnostics (Mannheim, Germany).