Sentence examples for blocking reactions were performed from inspiring English sources

The 3'-end blocking reactions were performed for 1 hr at 37°C and stopped by denaturing the enzyme at 70°C for 20 minutes.

Sections of 7 µm thickness were cut from the formalin-fixed, paraffin-embedded spinal cord blocks, and immunohistochemical reactions were performed.

γ-OH-PdG posed a significant block to replication, and when the reactions were performed with individual deoxynucleotide triphosphates using −1 primers (the 3′ primer terminus was positioned immediately prior to the adducted site), incorporation opposite the adduct was most efficient in the presence of dGTP and dATP but not the correct dCTP (65, 66).

Blocking, addition of secondary antibody and washing reactions were performed according to the manufacturer's instructions for the BondMax staining system with Bond Polymer Refine Detection Reagents (Leica Microsystems, Wetzlar, Germany).

Various site-specific modification reactions were performed on biological mCRP to block or alter selected amino acid R groups prior to performing binding assays.

These reactions were performed in 24-well reaction blocks with automatic reagent dispensing under inert atmosphere.

These reactions were performed using 24 well reaction blocks and an automatic reagent-dispensing platform under inert atmosphere.

The reactions were performed as a plate experiment, employing an XT-block reactor, with seven organometallic reagents (M=MgX, Li, and ZnX) and ten amines.

Reactions were performed again using 90 cycles to maximize the chance that mispriming events with the blocked primers could be detected.

qPCR reactions were performed on an ABI 7900HT thermocycler equipped with a 384-well block in 5- μl-reaction mixtures containing 2.5  μl 2X SensiMix SYBR Hi-ROX (Bioline, London, UK), 2- μl DNA (10 ng) and 50-n M primers.

Mass spectrometry analyses of the reactions were performed qualitatively (with exception of labeling with NBS; see Experimental Procedures) which enabled determination of totally blocked residues only.