Sentence examples for blocking peptide for the from inspiring English sources

A blocking peptide for the Tiam1 antibody (Santa Cruz, sc872P) was used to validate the specificity of the antibody according to the manufacturer's protocols.

Blocking peptide for the IP3R1 antibody was used at the manufacturer's suggested concentration.

Controls included incubation in the absence of primary antibody and quenching of the primary antibody with the respective blocking peptide for 1 h at room temperature before application to the tissue.

For a negative control, the primary antibody was pre-incubated with the blocking peptide for 1 h at 25°C and centrifuged before being applied to tissue sections.

The primary antibody to COX-2 Catt. No. 160112) and the blocking peptide for COX-2 (Cat.No. 360107) were sourced from Cayman Chemical Company (Ann Arbor, Michigan, USA).

Specificity was checked by performing pre-adsorption of the primary antibody with the corresponding blocking peptide for 1 hour at 37°C.

Use of the blocking peptide for anti-GABAAα1 eliminated this staining (Figure 3E-F) which confirms that this staining pattern is specific for GABAAα1.

Nuclear staining for Snail was not seen in control sections in which a blocking peptide for Snail1 was included in the incubation (Figure 4B).

The blocking peptide for ATF3 IHC (sc-188p) was obtained from Santa Cruz (Santa Cruz, CA).

A blocking peptide for anti-GABAA Rα1 (corresponding to amino acids 28 43) was pre-incubated with the antibody (1 µg peptide with 1 µg antibody) and staining was eliminated.

To determine the specificity of staining, different control procedures were employed according to the same experimental protocol: first, the use of adsorbed immune serum (1 h, 37°C) with a 20-fold excess of human recombinant for MMP-13 protein (R&D Systems) and for 5-LOX protein (Cayman Chemical), or human blocking peptide for cathepsin K (Santa Cruz) and ADAMTS-4 (Triple Point Biologics Inc).