Sentence examples for blocking peptide at a from inspiring English sources

The immunostaining was completely abolished in S. canicula sections treated with primary antibodies at working dilution after pre-adsorption with the blocking peptide at a concentration of 10 mg/mL at 4 °C for 24 h.

For blocking peptide experiment intracellular targeting primary anti human Kirrel 3 antibody was incubated with blocking peptide at a ratio of 1 4 for 3 hrs at room temperature with gentle agitation.

For the peptide neutralization experiment, as part of the assay validation, the CRBN65 antibody was preincubated with a 10-fold (by mass) excess of blocking peptide at room temperature for 2 h with rotation before applying to the slides.

(F) Huh7.5.1 cells infected by HCVcc in the presence of antibodies or blocking peptide at 50 μg/mL at 37 °C for 48 h.

The sections were treated in the same manner as above except that COX-2 antibody was preincubated with 10 mcg/ml COX-2 blocking peptide at room temperature for one hour.

First, SC10432 was tested in all specimens after prior incubation with the corresponding blocking peptide SC10432P at 1 10 protein ratio versus PBS (Additional File 1: B).

To test the specificity of the PS1 antibody, a blocking peptide was used [19]; an excess of peptide (1.2 ng/µl) was incubated for 7 h with PS1 at 4°C before being incubated with the tissue sections.

For antibody specificity, goat anti-HIF-1α antibody was incubated with HIF-1α blocking peptide 1-1000; Satta CRTz) at RT for 2 hrs before staining the neurons for HIF-1α.

Briefly, primary antibody was incubated with 25× (by mass) blocking peptide in antibody diluents (at both 1 375 and 1 750) for one hour at room temperature before application to canine control and sample tumor slides.

Specificity of the anti-E2F-5 antibody was confirmed using an E2F-5 blocking peptide (SC-999 P, Santa Cruz Biotechnology) and by a single band at 59 kDa detected by immunoblotting.

For a negative control, the primary antibody was pre-incubated with the blocking peptide for 1 h at 25°C and centrifuged before being applied to tissue sections.