Sentence examples for blocking of unspecific binding from inspiring English sources
After the transfer, blocking of unspecific binding sites was achieved by incubation in TBST (50 mM Tris/HCl, 150 mM NaCl, 0.5% Tween 20, pH 7.2) containing 5% skimmed milk.
After washing the plates and blocking of unspecific binding sites, c-jun was detected using an ELISA detection reaction.
Cells (20 μl) were fixed to polylysine-coated glass slides and washed with PBS, followed by blocking of unspecific binding sites using 2% milk powder in PBS.
With improved fluorochrome labeling of dsDNA, removal of DNA aggregates, and enhanced blocking of unspecific binding, we were able to specifically detect dsDNA-reactive plasma cells by immunofluorescence microscopy.
For permeabilization and blocking of unspecific binding sites, colony pieces were first cut into smaller pieces and then treated overnight with a 2 4 % Triton-X solution containing 6%% normal goat serum in 0.1 M PB (Block PBT).
After blocking of unspecific binding sites with 1% BSA in PBS at room temperature for 1 h, staining with anti-SAMHD1 antibody (ProteinTech Group, Chicago, IL, USA) was performed.
Slides were washed 5 times with PBS prior to blocking of unspecific antibody binding with DAKO protein block serum-free (Dako, Hamburg, Germany) for 20 min at 37°C.
Blocking of unspecific bindings was done with FCS/Tris 20%.
For double-labeling with phalloidin and vinculin, the first labeling was performed with a 20-min incubation with Alexa 488 phalloidin (1 40 dilution; Invitrogen), followed by block of unspecific binding sites and then by overnight incubation with a monoclonal antibody to vinculin (1 100 dilution; Sigma-Aldrich).
