Sentence examples for blocking of the splice from inspiring English sources

Morpholino oligomers can also be used for efficient blocking of the splice machinery [ 5].

The system was originally established by Kole and his colleagues to identify AO sequences for effective blocking of the aberrant splicing site in the mutated β-globin gene [28].

The generation of E10− RNA is not the consequence of a direct change in cis-splicing activity secondary to the blocking of a potential splicing enhancer in intron 9 by the binding of the PTM as such products were not observed with TauPTM6 which has the same binding domain as TauPTM9 (Fig.  4D).

Exon inclusion can be achieved by blocking of intronic splicing silencer sequences as for the treatment of SMA.

Reverse transcription (RT -PCR analysis, peRT -PCR using primers on both sides of the splice site thanalysisrgeted by the morperformedprovided evidence of strong blocking of splicing, whereas overall morphology was not noticeably affected (Fig. 7A; the general morphology of the morphants is not shown).

No original 357-bp band was observed in the zdia2 sMO-treated embryos, which confirms the effectiveness of zdia2 sMO in blocking the splicing of zdia2.

This sequence is located immediately after the conserved sequence block B of the splicing domain.

To overcome the imbalance in the SMN2 splicing pattern, we designed antisense RNA oligonucleotides to block the 3' splice site of SMN exon 8.

This splicing substrate goes through the first step of chemistry, yielding a cleaved 5' exon and lariat-intron intermediate, but the second step is blocked due to the 3' splice site mutation, and a population of C complex spliceosomes accumulates.

More interestingly, there was a higher rate of severe phenotype by translation blocking morpholino than the splicing blocker.

These results suggest that selective blocking of pro-angiogenic isoforms by inhibiting splice-site selection with SRPK1 inhibitors reduces melanoma growth.