Sentence examples for blocking of sections from inspiring English sources

To improve antibody penetration of the tissue, the sections were incubated for 1 h in 0.2% Triton X-100, followed by blocking of sections with 5% goat serum in PBS to prevent non-specific binding.

Blocking of sections was done with PBS/0.3% Tween-20/2 Tween-20/2inally incubated for 1 h in the presence of anti-α-tubulin DM1-A (Sigma, Missouri, USA) (1∶100), as a primary antibody and with anti-mouse IgG conjugated to FITC (ZYMED, California, USA) as secondary antibody (1∶30).

After deparaffinisation and blocking of tissue sections, primary goat polyclonal antibody against MCAK (Abcam, Cambridge, UK) was applied at a 1 : 100 dilution, and the antigen antibody reaction was incubated overnight at 4°C.

After blocking of endogenous peroxidase, sections were incubated with rabbit antiserum to HEX diluted 1 250 in PBS overnight at 4°C.

Following dewaxing and blocking of endogenous peroxidase activity sections were pretreated for antigen retrieval (Table  3).

Small blocks of sections with anterograde, NR1 or mGluR1a and CB or PV label were cut under a dissecting microscope, postfixed in 1% osmium tetroxide with 1.5% potassium ferrocyanide in PB, washed in buffer (PB) and water, and dehydrated in an ascending series of alcohols.

In its ruling today, the court did not strike down the website blocking powers of Section 66A, however, according to DNA.

Those who are not in the squares and yards block off sections of the street with slabs of concrete and sleep right on the pavement.

The action asks the court to block enforcement of Section 2(B) of S.B. 1070, the notorious Arizona law that compels all law enforcement agencies in the state to copy and carry out the Arpaio model of harassment and humiliation.

"There were already protesters lining up and blocking sections of the freeway in LA.

After removal of blocking buffer, sections were incubated at 4°C overnight with fluorescein-reporter conjugated and Biotin conjugated c-Met antibodies prepared in blocking buffer.