Exact(9)
In light of these results it becomes important to assess the outcome of blocking membrane insertion at the plasma membrane.
This suggests that the CtrIncA N-terminal SNARE-like motif requires a SNARE-like motif of at least ∼23 amino acids in order to exert an effective inhibitory activity and confirm the role of this motif in blocking membrane fusion.
Immunoblotting was performed using TBS Tween (0.1%), containing 5% nonfat dry milk for blocking membrane and for antibody solutions.
Immunoblotting was accomplished with TBS Tween (0.1%), containing 5% nonfat dry milk for blocking membrane and for antibody solutions.
Attempts to inhibit tumor growth by blocking membrane association of signaling proteins have been pursued over the years [ 1].
This indicates that blocking membrane GABA reuptake does not immediately interfere with the ability of dopaminergic terminals to release GABA, presumably because synaptic vesicles are already filled and cytoplasmic GABA levels have not yet been depleted.
Similar(51)
Following blocking, membranes were probed with various primary antibodies to determine different levels of protein expressions.
After blocking, membranes were exposed to peroxidase-conjugated anti-myc (Santa Cruz) and blots were developed using chemiluminescence.
After blocking, membranes were probed with the indicated antibodies, followed by an IRDye700-conjugated secondary antibody of the appropriate species (LI-COR, Lincoln, NE, USA).
After blocking, membranes were hybridized with respective primary antibodies.
After blocking, membranes were incubated overnight at 4°C in blocking solution containing primary antibody.
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