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The blocking medium was then replaced with zinc-adequate or zinc-deficient medium to allow cells to progress in the cell cycle, and cells were harvested at different times thereafter.
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After 2 h at 37°C, the blocking medium was discarded.
The behaviour of such block copolymers in aqueous medium was then investigated in relation to the respective size of both the amphiphilic and the hydrophobic block.
The medium was then adjusted at pH 6.9.
The medium was then adjusted at pH 6.5.
Cell culture medium was then replaced with CNT-modified medium.
The medium was then allowed for solidification.
The medium was then changed daily.
Standard growth medium was then added.
The medium was then changed to serum-free medium.
The medium was then removed and replaced with fresh medium.
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