Sentence examples for blocking medium was removed from inspiring English sources

Transwell inserts and lower wells were blocked with 1% BSA/PBS overnight at 4 °C; thereafter, blocking medium was removed and lower wells were loaded with 300  μl of fMLF, fMMYALF or fMLKLIV in DMEM+0.5% BSA in concentrations ranging from 10−7 ℳ to 10−10 ℳ. U87 or U937-FPR1 cells were serum starved for 2 days.

After 2 h at 37°C, the blocking medium was discarded.

After incubation for 18 h at 37°C, the culture medium was removed, the slides were washed, blocked, reacted with the mAbs and observed as described above.

After 24 h, the medium was removed, and free thiols were blocked by incubation with 20 mM NEM (N-ethylmaleimide) (Sigma) in PBS for 2 min on ice.

After treatments, culture medium was removed and washed with PBS.

Every 2 3 days, medium was removed, cells were washed with PBS (Gibco) and fresh medium was added.

Then the growth medium was removed from cells and replaced with 3 mL of complete growth medium.

After 24h of incubation, the cell culture medium was removed.

The old medium was removed and subsequently the fresh medium was added with 100 μL/well.

The culture medium was removed and the cells were washed with the medium.

For measuring cell-associated luciferase activity, culturing medium was removed.