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Signatures for different PEG tags have consistent patterns and distinct blocking levels; thus PEG is an optimal barcode motif.
Furthermore, reduced or blocking levels of CD4 expression, can convert T cell responses from stimulatory to inhibitory 48.
Figure 3 a indicates that using probes with the same PEG tag to detect different miRNAs resulted in similar blocking levels.
Indeed, their signatures in Figure 3 b clearly reveal well-separated blocking levels, allowing almost error-free discrimination of four miRNAs.
This is consistent with previous findings that different length PEGs or PEG-labeled nucleotides can be discriminated by blocking levels at a resolution of 1.6 pA per PEG unit (4 M KCl, +40 mV).
For example, P8 tagged between C5/C6 along the probe close to the pore opening, rather than C2/C3 in the sensing zone, can no longer generate the characteristic stage Ib, making it impossible to discriminate blocking levels by differently tagged probes.
Similar(53)
Each stage is at a specific blocking level.
These signatures are different not only in blocking level but also in duration.
This finding suggests the possibility of using PEG to program the stage Ib blocking level.
Use the dropdown menu at the bottom to choose your blocking level.
Move the slider to the right or left to decide the blocking level.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com