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Fast and highly efficient surface blocking is achieved avoiding the necessity of additional blocking steps.
It is established that, for a given ratio Et/Ef of the elastic moduli in the transverse and fiber directions, there exists a critical facesheet thickness above which crack blocking is achieved and crack growth is prevented.
Similar(58)
After transference, membrane blocking was achieved by incubation in AP-T buffer (1 M Tris/HCl pH 7.4, 1 M NaCl, 25 mM MgCl2, 0.03% (v/v) Tween 20) containing 5% (w/v) milk powder.
Protein blocking was achieved by a 20 min incubation in PBS (Phosphate Buffer Saline) with 1% of horse serum and 5% human normal serum (blocking serum).
Unexpectedly, a stricter enhancer blocking was achieved by CTCF alone than by a combination of the CTCF, USF1 and VEZF1 sites, illustrated by undetectable Igf2 expression upon paternal transmission.
Cells were permeabilized with 2% saponin in PBS, blocking was achieved by incubation with 0.5% bovine serum albumin in PBS.
Further blocking was achieved through exposure to normal rabbit serum (diluted with TBS) for 30 min at room temperature (20 22°C).
Further blocking was achieved through exposure to normal rabbit serum (diluted with TBS) for 30 min at room temperature.
Endogenous avidin and biotin were blocked using the Vector Avidin/Biotin Blocking Kit (Vector Laboratories, Inc., Burlingame, CA), and further blocking was achieved using swine serum.
In the SN and SD groups, anaesthesia was induced with propofol at 1.5 2.0 mg kg−1 and manual mask ventilation was done with 3 4 vol% sevoflurane and 50%% O2-air mixture until adequate neuromuscular blocking was achieved.
When the two peptides Cit573 and Cit591 were equally mixed and tested in the competition assay, a significantly higher degree of ACPA blocking was achieved, reaching 91 % inhibition (data not shown).
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