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For specificity, control blocking experiments were run with unlabelled specific double-stranded oligonucleotides or a respective mutant variant (5' -CatCAGAGGCGACTTTCCGAGGGATG-3') at 10-fold and 100-fold molar excess of the labelled NF-κB probe.
Similar(59)
The 45 experiments were run in 5 blocks of 9 experiments each (Table 2) and RSM was applied to fit the second order polynomial equation (4) to the experimental results as shown in Table 2.
The experiments were run 1000 times for each block length, with the presented results the average on the trials.
Four sets of experiments were run.
Two independent experiments were run.
Experiments were run using custom software.
Three different experiments were run (Table 1).
Experiments were run by DB.
Experiments were run three times.
Experiments were run in triplicate.
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