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C-Kit blocking experiments were performed by intraperitoneal injections of anti-c-Kit or appropriate isotype control (50 μg) on daily for commencing from day 10.
Isotype controls or phosphopeptide blocking experiments were performed to characterize each phospho-antibody.
In order to investigate the in vivo specificity, blocking experiments were performed.
In the present studies using mice, the cold-displacement and blocking experiments were performed using the co-injection method.
To determine the relative importance of CD36 and SR-A in the uptake of AcLDL in db/+and db/db PerMΦs ex vivo antibody-dependent blocking experiments were performed.
Antibody blocking experiments were performed by preincubating primary antibody with approximately 2 μg of GST-Evc2 on beads and GST control (for R1656).
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An additional blocking experiment was performed by co-administration of compound 2 (50 μg, 75 nmol) and [18F]2, which corresponded with a molar activity of 0.07 GBq · μmol−1.
Each blocking experiment was performed on two consecutive days using the same animal first studied with a baseline scan (control) followed by a PET scan after pre-treatment the animal with celecoxib (2 mg).
To determine whether laminin binding activity by LARGE-modified non-α-DG is sensitive to IIH6C4, a IIH6C4 blocking experiment was performed.
The blocking experiment was performed using RGD2 as the blocking agent.
To avoid non-specific binding, an immunizing peptide blocking experiment was performed.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com