Cocaine increases DA concentrations by blocking the DAT, and chronic drug use results in increased DAT levels and function that are thought to contribute to craving, withdrawal-induced anhedonia and binging.
By blocking the DAT, MPH may increase the tonic extracellular DA concentrations and thus decrease the phasic transmission [ 53].
In patients with attention deficit hyperactivity disorder (ADHD), stimulant medications (i.e amphetamine and methylphenidate) that block DAT and enhance DA signaling are widely used therapeutically to improve attention [2], [14].
BBC no fit confam if na true-true say di security pipo wey di for dia vans, dey really block di road so dat im no go fit comot for house.
Many therapies designed to reduce food intake and body weight act, in part, by blocking the dopamine transporter (DAT) — a protein responsible for clearing extracellular dopamine (DA) after release thereby terminating its action.
Plasmalemmal DA transport into samples prepared from frozen striata was blocked by the DAT inhibitor, cocaine, and vesicular DA transport was blocked by the VMAT-2 inhibitor, dihydrotetrabenazine. Additionally, K+-stimulated DA release was Ca+2-dependent.
Sections were incubated overnight at 4°C with antibodies against DAT (1∶30,Chemicon, CA) and TH (1∶40 Chemicon, CA) in blocking media.
Triple reuptake inhibitors (TRIs) that block the dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) are being developed as a new class of antidepressant that may have better efficacy and fewer side effects compared with traditional antidepressants.
