Sentence examples for blocking buffer were transferred from inspiring English sources

Phage (1 × 10) carrying different peptide sequences in 100 μl blocking buffer were transferred to the coated wells and incubated at 37°C for 1 h.

After permeabilisation in PBS/0.25% Tween for 5 minutes at room temperature, oocytes were transferred to blocking buffer (containing PBS/2% bovine serum albumine (BSA)/2% normal goat serum/0.1 M glycine/0.01% Triton X-100) and incubated at 4° overnight.

Proteins were transferred to a PVDF membrane and blocked overnight in blocking buffer (Sigma-Aldrich).

Gels were transferred to nitrocellulose membranes and blocked overnight at 4°C in blocking buffer (PBS, 0.05% Tween-20, 10% normal goat serum, 0.05% non-fat dry milk).

After separation by electrophoresis, proteins were transferred to nitrocellulose membranes and incubated with blocking buffer as described previously [12].

After separation by electrophoresis, proteins were transferred to nitrocellulose membranes and incubated with blocking buffer as described previously (McClintock et al., 2006).

Proteins were transferred to nitrocellulose membranes (WhatmanProtran, GE Healthcare) and membranes were blocked with blocking buffer (TBS-Tween buffer containing 5% milk).

Gels were transferred to PVDF membranes, blocked in Odyssey buffer (Li-COR #927-40000) for 1 hour and incubated with primary antibodies overnight.

Separated proteins were transferred in 20% Tris-methanol buffer onto a nitrocellulose membrane (Pierce) and blocked with Li-Cor blocking buffer (Biochrom) diluted 1 1 with PBS (Biochrom) for 2 hours.

Following electrophoresis, the proteins were transferred to nitrocellulose membranes by the iBlot device (Invitrogen), blocked with an Odyssey blocking buffer (LI-COR Biotechnology) for 1 h.

Gels were transferred to PVDF, and the membranes were incubated with infrared blocking buffer (Rockland Immunochemicals) for 1 h at RT.