Sentence examples for blocking buffer were loaded from inspiring English sources

Human IgG standard (Sigma) and culture supernatants diluted with a blocking buffer were loaded into each well and treated with peroxidase conjugated goat anti-human IgG (Sigma) in the blocking buffer.

ChonBlock™ blocking buffer was procured from Chondrex, Inc. (Redmond, WA).

Before loading on the microarray, the hybridization solution was heated for 3 minutes at 100°C and 5.8 μl of 10 × DIG blocking buffer was added.

After blocking, slides were loaded to the autostainer.

Blocking buffer was used to avoid non specific binding.

A series of 1 2 dilutions of the different serum samples, starting with a 1 400 dilution, were loaded onto the antigen-coated plates in the blocking buffer and then incubated for 1 h at room temperature.

Peptides were loaded onto the column with buffer A (0.1% FA) and were eluted with 300 nL min−1 buffer B (99.9% ACN, 0.1% FA).

A prepared PVDF membrane (0.45µ, Millipore) were loaded to different samples from the concentrated supernatants or the purified solutions, and then blocked by PBS blocking buffer (Pierce) for 1 hour with gentle agitation.

Samples were loaded with Orange G loading buffer (4% sucrose in dH20 + 0.01% Orange G).

Cell lysates (25 µg) were loaded on SDS PAGE 10% gels and proteins transferred to PVDF membrane and blocked in TBS buffer containing 1% Tween 20 and 3% (W/V) BSA.

Volumetric images were loaded into memory as ≈ 512 pixel blocks.