Sentence examples for blocking buffer were added from inspiring English sources

After blocking with 200 µl 1% BSA in PBST (phosphate buffered saline, pH 7.6, containing 0.05% Tween-20), 100 µl of prepared antibodies gradient diluted in blocking buffer were added and incubated at 37°C for 2 h.

After washes, 50 µL of different concentrations of recombinant Hsp60 (ranging ranging from 20 µg/mL to 0.16 µg/mL in a 1∶2 serial dilution in blocking buffer were added to each well.

Fluorescent secondary antibodies in blocking buffer were added and incubated for 1 hr at RT, followed by three washes in PBS for 5 min. Cells were visualized with a Olympus IX71 microscope with a CoolSnapHQ camera (Roper Scientific, Tucson/AZ, USA).

Samples diluted in blocking buffer were added to each well and incubated at 4°C overnight.

Primary antibodies diluted in blocking buffer were added and incubated over night at 4°C.

Alexa Fluor-conjugated secondary antibodies, diluted 1 200 in blocking buffer, were added for 1 hour at room temperature.

Rabbit anti-LukF-PVL anti-serum (1∶20,000 in the blocking buffer) was added to each well.

Briefly, the provided blocking buffer was added to array membranes for 30 minutes.

Two milliliters of 2% BSA blocking buffer was added to each tube, and tubes were rinsed by centrifugation at 350 g.

An equal volume of A1 moAb diluted 1∶5,000 in blocking buffer was added to each standard or sample dilution, and these mixtures were incubated overnight at 4°C.

For the detection of IgA antibodies, 100 µl of goat anti-monkey IgA (Nordic Immunology, Tilburg, Netherlands) diluted 1∶5000 in blocking buffer was added to each well.