Sentence examples for blocking buffer was then from inspiring English sources

Detection antibody (anti-rabbit UCH-L1-HRP conjugation, made in-house at 50 μg/mL) in blocking buffer was then added to wells at 100 μl/well and the plates were further incubated for 1.5 hours at room temperature.

Plates were washed with PBS, 0.1% Tween 20 (v/v), and then blocked with PBS, 0.1% Tween 20, and 2% nonfat dried milk (w/v) for 1 h at 37°C. 10 TU of individual phages was isolated after third panning and 50  μL of blocking buffer was then added to each well.

Serial dilutions of Rad51 in blocking buffer were then added to the appropriate wells of the ELISA plate and incubated for 1 h at 30°C.

Serum samples (diluted 1 in 110 in a blocking buffer) were then added to the plates and allowed to incubate at room temperature with shaking for 90 min. Following incubation, plates were washed and horseradish peroxidase (HRP -conjugated rabbit anti-HRP -conjugated, Glostrabbitenmark) was added.

Serum samples (diluted 1 in 110 in a blocking buffer) were then added to the plates and allowed to incubate at room temperature with shaking for 90 min. Following incubation, plates were washed and horseradish peroxidase-conjugated rabbit anti-human IgG (Dako, Glostrup, Denmark) was added.

The sections were then incubated with a blocking buffer (1% blocking buffer and 1X maleic acid buffer, DIG Wash and Block Buffer Set, (Roche Diagnostics, Mannheim, Germany) for 60 min. 40 μl of anti-DIG antibodies (Roche Diagnostics, Mannheim, Germany) diluted 1 2000 using blocking buffer was added to each section and then slides were incubated overnight at 4°C.

Either rat anti-mouse CD31 or isotypic antibodies 1∶100 in 10× diluted blocking buffer were applied for 1.5 h and then sections were washed with PBS.

Superblock T20 PBS blocking buffer (Pierce) was then added for 1 h at room temperature (RT) with shaking at 150 rpm.

The blocking buffer then was replaced with HRP-6B6C.1 antibody diluted in blocking buffer and incubated for 1 h at room temperature.

After treating 30 min to 1 hr in Odyssey blocking buffer, the membranes were then incubated for 1 hr at RT with 1 10,000 of anti-PGK using the same IgG-HRP-secondary antibody as described.

Blocking of membranes was then done in blocking buffer (Hepes buffered saline containing 0.1% Triton X-100, 1% BSA, 1% fish gelatin and 5 mM EDTA).