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The blocking buffer was tapped off and washed as described above before the addition of 100 μl of serum (1 100 diluted in blocking buffer).
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ChonBlock™ blocking buffer was procured from Chondrex, Inc. (Redmond, WA).
Subsequently, 5 % normal goat serum blocking buffer was applied.
The blocking buffer was decanted from each container.
The blocking buffer was discarded, and the primary antibody preparation (rabbit anti-CBM, diluted 1 3000 in blocking buffer) was added.
Blocking buffer was used to avoid non specific binding.
Blocking buffer was used to dilute primary and secondary antibodies.
The DHS blade was tapped into each test block to a depth of 30 mm as per manufacturers' instructions.
The blocks were tapped by the experimenter in randomized sequences of increasing length.
Unspecific binding was blocked in blocking buffer (LICOR).
Nonspecific binding was blocked with blocking buffer containing 3% BSA.
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