Sentence examples for blocking buffer was supplemented from inspiring English sources

For membranes utilized for phospho-Smad2 detection the blocking buffer was supplemented with sodium fluoride (50 mM).

For primary antibody incubations, the blocking buffer was supplemented with 0.1%Tween-20Tween-20or secondary antibody incubations, 0.1% Tween-20 and 0.01% SDS were added.

To block phosphatases in membrane fractions, permeabilization buffer was supplemented with either 2 mM activated sodium orthovanadate (Na3VO4) alone, or a cocktail of phosphatase inhibitors (5 mM β glycerophosphate, 1 mM sodium molybdate, 2 mM sodium pyrophosphate, 10 mM sodium fluoride and 2 mM activated Na3VO4 (each from Sigma-Aldrich)).

When optical tweezers were used, the ATP buffer was supplemented with NeutrAvidin-functionalized silica microspheres.

Wild-type and mutant FICD/HYPE were expressed and purified as EfFIC, except that the lysis buffer was supplemented with 1 mM dithiothreitol (DTT) and 0.02 % Triton X-100 and the other buffers were supplemented with 1 mM DTT.

AP1 lysis buffer was supplemented with 2.5%PVP400.

Buffer was supplemented with 100 μM FAD where indicated.

General actin buffer was supplemented with 0.2 mM final ATP.

The buffer was supplemented with 1 mM calcium chloride.

Labeling kit assay buffer was supplemented with 2% FBS.

Lysis and wash buffer were supplemented with 5 mM imidazole and elution buffer supplemented with 300 mM imidazole.