Sentence examples for blocking buffer was replaced from inspiring English sources

The blocking buffer was replaced with 100 µl of zipper-scFv G28-5 prorein or 293T cell lysates containing zipper-modified fiber proteins diluted in binding buffer (TBS with 0.05% Tween 20 and 0.05% casein).

The blocking buffer was replaced with binding buffer containing labeled RNA and microarray slides incubated in the dark at 25°C for 1 hour with gentle agitation.

The blocking buffer was replaced with an appropriate dilution of primary antibody in odyssey buffer with 10% PBS and incubation was continued at 4°C over night with gentle shaking.

ChonBlock™ blocking buffer was procured from Chondrex, Inc. (Redmond, WA).

Subsequently, 5 % normal goat serum blocking buffer was applied.

The blocking buffer was decanted from each container.

Permeabilisation buffer was replaced with 1 mL of cold blocking buffer (0.1 % Triton-X, 0.2 % skimmed dry milk in PBS) at 4 °C overnight.

After the formation of GUVs, the outer buffer was replaced by a fresh buffer-1 containing 200 mM glucose.

The buffer was replaced after each measurement to avoid saturation of the oil in the incubating buffer.

For buffer desalting, the ribosome dilution buffer was replaced with PBS through ultrafiltration (Amicon Ultra 3000 Da MW; Merck, Darmstadt, Germany).

Buffer was replaced every second day in all cases.