Sentence examples for blocking buffer was removed from inspiring English sources

After blocking buffer was removed, primary antibody was applied on the sections.

The blocking buffer was removed, and 200 μL of the azide-modified proteins were added to the beads for 2 h.

The blocking buffer was removed after 1 h of incubation in humidified chamber and primary antibody was added to the slides, incubated overnight at 4 °C.

The blocking buffer was removed, and the plates were washed and incubated with a solution of 1  μg/mL of fibronectin (Sigma) in IB buffer by 1 h at 37°C.

After 10 minutes, the blocking buffer was removed and the cells were incubated in 100 μl M30 CytoDeath antibody (1 100; Boehringer Ingelheim, France) at room temperature for 60 minutes.

The blocking buffer was removed and monoclonal antibodies (Mab) specific for human TRAIL receptors TR1, TR2, TR3, TR4 (as above), OPG (Mab 8051 or isotype-matched nonbinding control Mabs (as above), each diluted to 10  μg ml−1 in blocking buffer as above, were added for 60 min.

Tris-NaCl-blocking buffer was removed and replaced with anti-human RANK N-2B10.1 N-2B10.18.1; Amgen, Seattle, WA) or RANKL (M366; Amgen, Seattle, WAmgenuSeattleclonal antibodies or isotype-matched control mouse IgG (BD Pharmingen, San Jose, CA) at concentrations of 5 μg/mL for anti-RANK and 1 μg/mL for anti-RANKL for 60 minutes.

The blocking buffer was subsequently removed and the plate was washed with PBS-T three times.

The blocking reagent was removed and rabbit anti-HCC (diluted 1 1000 in blocking buffer) was added and incubated for 3 h at 37°C.

The 5.3 kb ssDNA (0.5 pM) in the blocking buffer was incubated in the flow chamber for 10 min and unattached DNA was removed by extensive washing as described for the FRET studies.

ChonBlock™ blocking buffer was procured from Chondrex, Inc. (Redmond, WA).