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ChonBlock™ blocking buffer was procured from Chondrex, Inc. (Redmond, WA).
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Subsequently, 5 % normal goat serum blocking buffer was applied.
The blocking buffer was decanted from each container.
All the medium solutions and buffers were procured from Quality Biological Inc. (Gaithersburg, MD).
Blocking buffer was used to avoid non specific binding.
A list of all the health centres in the five identified blocks was procured.
Cell lysis buffer for Western was procured from Beyotime Institute of Biotechnology, China.
Poly(lactic-co-glycolic acid) (PLGA) 50 50 block copolymer (RG 503H, molecular weight 35 40 kDa) was procured from Boehringer Ingelheim KG (Ingelheim, Germany).
Reducing sample buffer containing 100 mM dithiotreitol as well as a detection kit for enhanced chemiluminescence (ECL SuperSignal West Pico Substrate) was procured by Thermo Fisher Scientific (Dreieich, Germany).
A flashlight was procured.
No funding was procured for this work.
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