Sentence examples for blocking buffer was performed from inspiring English sources

Incubation with primary antibodies diluted in blocking buffer was performed overnight at room temperature (RT).

The first antibody (anti-EmIR2, 1 10 in blocking buffer) was added and incubation was performed overnight at 4°C (humid chamber).

The washes were performed between each step and the blocking buffer was used for dilution of each of the reagents that were overlaid onto the tissue sections.

ChonBlock™ blocking buffer was procured from Chondrex, Inc. (Redmond, WA).

Subsequently, 5 % normal goat serum blocking buffer was applied.

Heat induced antigen retrieval in pH 9.0 EDTA buffer was performed and samples were blocked with peroxide blocking solution from DAKO DAKOO, Hamburg, Germany).

19, 20 Antigen retrieval using citrate buffer was performed on dewaxed sections prior to blocking with swine serum.

Incubation with a mouse monoclonal primary antibody targeting E-cadherin (BD, San Jose, CA, USA Biosciences, 610181) in blocking buffer (1 100) was performed O/N at 4°C followed by three washes for 5 min in 0.3% Triton X-100 in PBS.

After incubation in blocking buffer, antibody staining was performed as per standard procedures.

Following the EdU detection reaction, larvae were incubated in blocking buffer and immunostaining was performed as described above.

Primary antibodies were diluted in blocking buffer and incubation was performed overnight at 4°C (cyclin D1 and p21 1:500 (Cell Signaling); cyclin E (sc-481) 1 100 (Santa Cruz); CDKN2A 1 200 (Thermo Scientific)).