Sentence examples for blocking buffer was incubated from inspiring English sources

Next, 100 µL of 0.1% ChonBlock™ blocking buffer was incubated with the probe antibody-conjugated microbeads with vortexing at 1400 rpm for 2 h at room temperature.

The 5.3 kb ssDNA (0.5 pM) in the blocking buffer was incubated in the flow chamber for 10 min and unattached DNA was removed by extensive washing as described for the FRET studies.

The secondary antibody (1 5000 in blocking buffer) was incubated at 25°C for 1 h before standard enhanced chemiluminescence detection.

After washing, peroxidase-conjugated anti-mouse IgG (diluted 1 : 1000 in the blocking buffer) was incubated for 60 min at 37°C.

Samples were then washed three times for five minutes with PBS and the second antibody (POX-anti-rabbit, 1 50 in blocking buffer) was incubated for three hours at room temperature in a humid chamber.

After four washes with PBS-T 0.05 %, HRP-conjugated rabbit anti-human IgG (Roche Applied Science) diluted (1 5,000) in the blocking buffer was incubated for 1 hour at 37 °C under gentle agitation.

One hundred µL of IgG at a concentration of 0.0205 g/L and diluted in blocking buffer were incubated for 90 min at 18°C.

After blocking with PBS containing 0.05% Tween 20 and 0.25% bovine serum albumin (BSA), pH 7.4 (blocking buffer), serial dilutions of patient serum in blocking buffer were incubated for 4 h at room temperature.

Plasma samples diluted 1 2,500 with dilution buffer (30 μL/well) or standards (30 μL/well) along with 10 μL of biotinylated antigens in blocking buffer were incubated with 10 μl of pre-mixed bead sets into the wells of a pre-wet 96-well microtitre plate.

Primary antibodies diluted 1 1000 in Odyssey Blocking Buffer were incubated with membranes at room temperature for 2 h.

Streptavidin conjugated horseradish peroxidase (DAKO, Osaka, Japan) in blocking buffer was mounted and incubated for 1 h at RT.