Sentence examples for blocking buffer was employed from inspiring English sources

Alkaline phosphatase-conjugated goat anti-mouse Fab specific fragment (Sigma-Aldrich, St . Louis MO, USA) diluted 1 5000 with blocking buffer was employed as secondary antibody.

ChonBlock™ blocking buffer was procured from Chondrex, Inc. (Redmond, WA).

Subsequently, 5 % normal goat serum blocking buffer was applied.

The blocking buffer was decanted from each container.

The blocking buffer was discarded, and the primary antibody preparation (rabbit anti-CBM, diluted 1 3000 in blocking buffer) was added.

In our hands, Odyssey blocking buffer is the ideal blocking reagent to consistently minimize background.

Blocking buffer was used to avoid non specific binding.

Blocking buffer was used to dilute primary and secondary antibodies.

No other blocking agents were employed.

Standard ORNL buffer layer architecture (CeO2/YSZ/CeO2) was employed as well.

Randomized complete block design with three replicates was employed.