Sentence examples for blocking buffer was discarded from inspiring English sources

The blocking buffer was discarded, and the primary antibody preparation (rabbit anti-CBM, diluted 1 3000 in blocking buffer) was added.

Blocking buffer was discarded and 100 μl of diluted serum was added to each well (dilution 1 200).

ChonBlock™ blocking buffer was procured from Chondrex, Inc. (Redmond, WA).

Blocking buffer was used to avoid non specific binding.

After 40 minutes, the blocking solution was discarded and the membrane was incubated with a binding buffer, Solution F. After washing, the chemiluminescent substrate was incubated with the membrane for two to five minutes at room temperature.

After 2 h at 37°C, the blocking medium was discarded.

The blocking serum was discarded and then the primary antibodies were added directly.

After initial blocking, the volume was discarded and 100 µl of blocking solution was added to each well.

Tissues were thawed on ice, and the original buffer solution was discarded.

The coating solution was discarded and blocking buffer (TBS (Tris buffer saline composed of Tris 3 g/l, NaCl 8 g/l, KCl 0.2 g/l adjusted to pH 7.4), 10 mM CaCl2, 0.05% Tween 20, 2% BSA) was added (1 hour incubation).

The first block of each condition was discarded in the analysis since it functioned as a training block.