Sentence examples for blocking buffer was aspirated from inspiring English sources

The following day, the blocking buffer was aspirated and washed in PBS-T.

Antibody in blocking buffer was aspirated and embryos were washed in 4 15 minute PBT washes at RT.

After 2 h, the blocking buffer was aspirated and serum (1 20 dilution in 0.5% Casein/PBS, pH 7.4) was added to the plate (50 μl/well).

ChonBlock™ blocking buffer was procured from Chondrex, Inc. (Redmond, WA).

The blocking buffer was decanted from each container.

Subsequently, 5 % normal goat serum blocking buffer was applied.

The blocking buffer was discarded, and the primary antibody preparation (rabbit anti-CBM, diluted 1 3000 in blocking buffer) was added.

Krebs buffer was aspirated, and the cells were incubated with 300 μL of [18F]DCFPyL in 0.9 % NaCl (0.1 MBq ) for 60 min at 37 °C.

Loading buffer was aspirated and replaced with assay buffer.

Excess buffer was aspirated leaving approximately 50 μl of buffer and worms in each tube.

All free buffer was aspirated, and the wet antibody-HLA beads were immediately stored at −80 °C.