Sentence examples for blocking buffer to a from inspiring English sources

GST-PPIL1 fusion protein or GST alone were diluted in blocking buffer to a final concentration of 40 µg/ml.

The Cy3-labelled streptavidin (Sigma, S6402) was first diluted in the blocking buffer to a final concentration of 20 µg/ml (0.33 µM).

In brief, specific antibodies were diluted in blocking buffer to a dilution of 1 100 1 400.

For sandwich ELISA, samples were diluted in blocking buffer to a final volume of 50 μl, added to the plate and incubated for 2 h.

After 5 washes with distilled water, triplicate wells were incubated at 37°C for 1 hr with various human sera samples diluted (1 1600) in blocking buffer to a volume of 100 μl.

Proteins were diluted in blocking buffer to 0.1 μM.

Primary antibody solutions (1 200 rabbit anti-cleaved lamin A in blocking buffer for apoptosis; 1 900 mouse anti-phospho-histone H3 in blocking buffer; 1 100 rabbit anti-alpha-tubulin in blocking buffer to show cellular morphology) were applied overnight at 4°C.

Samples and controls were diluted in blocking buffer to fit the standard curve range.

A HRP conjugated anti-HBsAg (AbD Serotec) was made in blocking buffer at a 1∶200 dilution.

Then, cells were fixed and blocked with 1X blocking buffer according to the manufacture's protocol.

The tissue was blocked using 5% goat serum 4% BSA blocking buffer according to standard protocol.