Sentence examples for blocking buffer for the from inspiring English sources

TA samples were diluted 1 400 to 1 6400 (in blocking buffer) for the secretory component assay.

At this step, an anti-DIG antibody conjugated with AP (1 1,000, Roche Diagnostics) was included in the 1% blocking buffer for the detection of the DIG probes.

After washing in TNT 3 times for 15 min, the sections were incubated overnight at 4 °C with an anti-DNP antibody conjugated with Alexa 488 (1 500, Molecular Probes, Eugene, OR) in 1% blocking buffer for the fluorescence detection of the DNP signals.

After washing with TNT buffer 3 times for 10 min, the sections were incubated overnight at 4 °C with an anti-DNP antibody conjugated with Alexa488 (1 500, Molecular Probes, Eugene, OR) in 1% blocking buffer for the fluorescence detection of the DNP signals.

After incubation with the blocking buffer for 1 h, the cells were incubated with the primary antibody AT8 (anti Phospho-PHF-tau detected phospho-S202, -T205) for 4 h, followed by three 5-min washes.

After blocking by the Western blocking buffer for 2 h the membranes incubated with primary antibodies of p-Syk, Syk and GAPDH at 4 °C overnight.

After incubation in blocking buffer for 1 h, the membranes were incubated with the primary antibodies specific for DNMT1, DNMT3a, DNMT3b, CTCF, RBP2 (Santa Cruz Biotechnology, Santa Cruz, CA) and β-actin (Cell Signaling, Danvers, MA).

After washing in TBS and incubation in blocking buffer for 10 min the sections were incubated with peroxidase-streptavidin for 30 min, and finally DAB was used as chromogen.

After introduction of ExtrAvidin peroxidase (1 : 10,000 dilution in blocking buffer) for 1 hour, the plates were developed with Sigma FAST OPD for 10 minutes.

Following washing cells were again incubated in blocking buffer for 30 minutes, before the addition of the secondary antibody (goat anti-mouse Alexa fluor 488, cat# A11001, Invitrogen) diluted 1∶500 in blocking buffer for 1 hour at 37°C.

Then, they were washed in phosphate-buffered saline for 10 minutes and incubated with blocking buffer for 1 hour before the slides were incubated overnight at 4°C with the primary antibody rabbit anti-BKCa channel diluted as above in blocking buffer or with PBS as a negative control.