Sentence examples for blocking buffer consisting of from inspiring English sources

Cells were washed again (three times with phosphate-buffered saline) and then incubated in blocking buffer consisting of phosphate-buffered saline supplemented with fetal bovine serum (5% v/v) and bovine serum albumin (1% w/v) for 1 h at 25°C.

Briefly, cultured PGCs were fixed in 3.7% paraformaldehyde solution for 10 min, washed three times with phosphate-buffered saline (PBS) and blocked with blocking buffer, consisting of PBS containing 5% (v/v) goat serum and 1% bovine serum albumin, for 30 min and then incubated with primary antibodies diluted 1∶200 in blocking buffer at 4°C overnight.

The membrane was blocked for 1 h in blocking buffer consisting of 5% non-fat skim milk/1X TBS-T (Tris-buffered saline with 0.1% Tween 20) for 1 h at room temperature, followed by incubation with primary monoclonal anti-Cyr61 antibody at 4°C overnight, diluted in 1X TBS-T (1 1000; R&D Systems, Minneapolis, MN, USA).

Sections were washed with PBS, and incubated for 15 min in blocking buffer consisting of 2.5% normal goat serum and 0.3% Triton X-100 in PBS.

Cells were blocked for 30 minutes at room temperature with blocking buffer consisting of PBS/T containing 1% BSA and 0.2% dried skim milk.

The blots were incubated at room temperature for 1 h in blocking buffer consisting of 5% (w/v) skim milk (Snow Brand, Sapporo, Japan) and 1× PBS containing 0.1% (v/v) Tween 20 (PBS-T).

Examination of sera was conducted using the Protein Binding Kit (Alere Technologies GmbH) with the following modifications: The incubation buffer P1 was supplemented with 1% (w/v) milk powder (Marvel; Premier International Foods UK Ltd ,Dublin, Ireland), and blocking buffer consisted of P1 containing 3% (w/v) milk powder.

Blocking buffer consisted of PBS containing either 10% donkey serum (Chemicon) or 0.2% Bovin Serum Albumin (Sigma-Aldrich) plus 0.05% saponin.

Blocking buffer consists of transcription buffer (10 mM HEPES (pH 7.6), 100 mM potassium glutamate, 10 mM magnesium acetate, 5 mM EGTA, 3.5% glycerol) containing 60 mg/ml casein, 5 mg/ml polyvinylpyrrolidone, and 2.5 mM DTT.

All antibodies were diluted in blocking buffer that consisted of 0.2 % non-fat dry milk, 2 % Bovine Serum Albumin and 0.3 % Triton X-100 in Phosphate Buffered Saline (PBS).

Transfer membranes were then blocked overnight with 5% w/v milk solution diluted in wash buffer consisting of PBS with 0.2% Triton X-100 (Sigma).