Sentence examples for blocking buffer composed of from inspiring English sources

The wells were then treated with a blocking buffer composed of 5% BSA in PBS and washed with buffer composed of 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, and 0.1% Tween 20.

For the bR synthesized in the PURE system, an assay buffer composed of 10 mM HEPES-KOH (pH 7.5), 5 mM MgCl2 and 100 mM KCl was used.

The placebo consisted of the viral formulation buffer, composed of sodium chloride, Tris buffer, trehalose (low endotoxin), magnesium chloride, monooleate (Tween 80) and water for injection.

The third scenario (randomized buffer) was a map of the core area plus a 25-km wide buffer composed of randomized habitat data (Figure 3c).

We recommend the use of a buffer composed of randomized habitat data as a solution to this problem.

Cells were suspended in buffer composed of PBS containing 1% FCS.

Blocking buffer was composed of PBST with 3% (w/v) BSA and 5% (w/v) sucrose.

"Noise buffers" composed of trees and shrubs may reduce noise by up to 15 db [ 81].

The coating solution was discarded and blocking buffer (TBS (Tris buffer saline composed of Tris 3 g/l, NaCl 8 g/l, KCl 0.2 g/l adjusted to pH 7.4), 10 mM CaCl2, 0.05% Tween 20, 2% BSA) was added (1 hour incubation).

Rinderpest and PPR blank controls (eight wells each) must be included, which receive only blocking buffer instead of antigen.

3. Add 40 m l of blocking buffer to all wells of the plate.