Sentence examples for blocking buffer at a from inspiring English sources

A HRP conjugated anti-HBsAg (AbD Serotec) was made in blocking buffer at a 1∶200 dilution.

Rabbit polyclonal antibodies against TGM-2 (Abcam, Cambridge, UK) and MMP-2 (Abbiotec, San Diego, CA), and mouse monoclonal antibody against CD24 (Neomarkers, Fremont, CA) were diluted with the blocking buffer at a factor of 1∶150, 1∶200, and 1∶100, respectively and incubated at 4°C overnight.

Cells were then washed twice with blocking buffer, and incubated with Alexa Fluor 488 goat anti-rabbit IgG (H + L) (Invitrogen) diluted in the blocking buffer at a concentration of 1 1,000 at room temperature for 1 h.

The membrane was incubated in Odyssey blocking buffer (LI-COR Biosciences, USA) at room temperature for 90 min, followed by incubation with a primary guinea pig polyclonal anti-VGLUT2 antibody (135404; Synaptic Systems, Germany) diluted with 5 ml Odyssey blocking buffer at a ratio of 1 1000 and 0.1 % Tween 20 (Sigma-Aldrich, USA) at 4 °C overnight with gentle rolling.

Permeabilized cells were blocked using a blocking buffer containing 1% (w/v) bovine serum albumin for 60 min followed by incubation in mouse anti-HA monoclonal antibody (Sigma) and rabbit anti-SCSα polyclonal serum in blocking buffer at a 1 1,000 dilution overnight at 4 °C.

A fluorescent-labelled secondary antibody, IRDye 680RD Donkey anti-guinea pig (LI-COR Biosciences, USA) diluted with Odyssey blocking buffer at a ratio of 1 10,000, supplemented with 0.01 % SDS, was then incubated with the membrane in the dark at room temperature for 45 min.

Membranes were incubated overnight with the primary antibody directed against TNF-β, TNF-α, MMP-9 and −13, Cox-2, p53, cleaved caspase 3, NF-κB-p65, TNF-β-R, PARP and β-actin at a 1 1,000 dilution in blocking buffer at 4°C on a shaker, washed three times with blocking buffer, then incubated with the secondary antibody conjugated with alkaline phosphatase for 90 minutes at ambient temperature (AT).

Brain sections were washed twice in 0.01 M PBS and incubated in blocking buffer (5% goat serum + 0.3% TritonX-100 in 0.01 M PBS) for 90 min at RT to block non-specific sites and incubated in primary antibody diluted in blocking buffer at 4°C overnight on a shaker.

Membranes pre-coated with cytokine antibody were blocked by a blocking buffer at RT for 30 min, then equal volumes of supernatants, collected from microglia pretreated with gp120 (Immunodiagnostic, Inc., Woburn, MA, USA) in the presence or absence of 4-AP or TEA, were added to replace the blocking buffer and incubated over night at 4°C.

Reactions were visualized by using a suitable secondary antibody conjugated to horseradish peroxidase (Dako) in blocking buffer at room temperature for 2 hours and an enhanced chemiluminescence system (Pierce).

Goat anti-rabbit Alexa 488 secondary antibody (Invitrogen) was added in blocking buffer at (10 μg/mL) and incubated for an additional hour at room temperature in the dark.