Sentence examples for blocking buffer and the from inspiring English sources

After 3 washes with PBS Tween 0.1%, the primary rabbit polyclonal antibody, produced using commercial imiglucerase (Genzyme Corp., Sanofi Aventis) as antigen, was diluted 1 1000 in the blocking buffer and the blot was incubated for 1 h at RT.

Primary antibodies were diluted in blocking buffer and the cells were incubated overnight.

For TGN46 staining, goat serum was omitted from the blocking buffer and the concentration of human IgG raised to 40 µg/ml.

After blocking with blocking buffer (LI-COR Biosciences), the recombinant HA were detected with an anti 6xHIS tag monoclonal antibody (Roche) diluted 1∶400 in blocking buffer and a polyclonal goat anti-mouse IRDye® 800CW conjugate (LI-COR Biosciences) diluted 1∶10.000 in blocking buffer and the Odyssey® Infrared Imaging System (LI-COR® Biosciences).

The primary antibodies were added, diluted in blocking buffer, and the cells incubated for 45 min at room temperature.

Bound antibodies were detected after washing using 50 ng ml−1 horseradish peroxidase-conjugated secondary antibodies in blocking buffer and the SuperSignal chemiluminescent detection reagent (Pierce, Weiskirchen, Germany).

Suspend the cells in 1mL of blocking buffer, and determine the number of live cells recovered.

Remove the antibody solution, wash three times with 200 µl PBS plus 0.05% Tween-20, add 50 µl anti-mouse or anti-rabbit HRP conjugated secondary antibody with 1∶1000 dilution in blocking buffer, and incubate the plate at room temperature for 1.5 hours.

After adding 100  μl of blocking buffer and washing the wells with PBST buffer, a condition medium of CCN2 transfectants collected for 48 h was added to separate wells.

The EDTA plasma samples were diluted 10 times in the blocking buffer and applied to the plates.

After the five washes, the competitor rabbit mAbs or sCD4 were diluted in the blocking buffer and added to the plates at three-fold serial dilutions, starting at 500 µg/mL.