Sentence examples for blocking buffer and stored from inspiring English sources

The beads were then resuspended in 200 μL blocking buffer and stored ON at 4 °C before use.

Antibodies and antiserum were diluted in blocking buffer and embryos incubated overnight in this solution at 4 °C.

Secondary antibodies were diluted in blocking buffer and applied to membranes for 1 h at room temperature.

Samples were then washed 3 × 30 minutes in blocking buffer and mounted on slides using Vectashield® (Vector Labs, Burlingame, CA).

Cells were subsequently washed three times in blocking buffer and incubated with the appropriate secondary antibody diluted in blocking buffer (donkey anti-rabbit AlexaFluor 647 or donkey anti-mouse AlexaFluor 647 (1:1000; Jackson ImmunoResearch)) for 2 hours at room temperature.

The cells were washed with the blocking buffer and passed through Cell Strainer (BD Biosciences, 40 µm pore size).

aureus antibody in blocking buffer, washed three times with blocking buffer, incubated for 20 minutes with 40 µg/ml Texas-RedX-conjugated goat anti-mouse IgG (Life Technologies) in blocking buffer, and washed twice with blocking buffer.

Cells were washed twice in blocking buffer and then incubated in 100 μl blocking buffer for 10 min with agitation.

Cortical ovarian pieces measuring 1 × 1 × 0.5 mm were fixed for 24 h at 4°C in microtubule stabilization buffer extraction fix (MTSB-XF) or 4% paraformaldehyde and samples were stored in blocking buffer (Rodrigues et al., 2009) at 4°C for 3 10 weeks prior to labelling.

The blocking buffer was discarded, and the primary antibody preparation (rabbit anti-CBM, diluted 1 3000 in blocking buffer) was added.

Slices were permeabilised by PBS-T (0.2% Triton-X 100 in PBS) for 30 minutes and blocked in blocking buffer (10% NGS and 5% BSA in PBS-T buffer) for 1 hr at room temperature.