Sentence examples for blocking buffer and plated from inspiring English sources

Following the transport assay, the cells were lysed in the blocking buffer and plated on ELISA plates.

Remove the antibody solution, wash three times with 200 µl PBS plus 0.05% Tween-20, add 50 µl anti-mouse or anti-rabbit HRP conjugated secondary antibody with 1∶1000 dilution in blocking buffer, and incubate the plate at room temperature for 1.5 hours.

Heat-inactivated plasma were serially diluted from 1∶50 to 1∶1600 in blocking buffer and placed on the plates for 1 h at RT. Plates were washed three times with PBST.

The EDTA plasma samples were diluted 10 times in the blocking buffer and applied to the plates.

After the five washes, the competitor rabbit mAbs or sCD4 were diluted in the blocking buffer and added to the plates at three-fold serial dilutions, starting at 500 µg/mL.

Sera were diluted with blocking buffer and incubated in 96 well plates at 4°C overnight.

A series of 1 2 dilutions of the different serum samples, starting with a 1 400 dilution, were loaded onto the antigen-coated plates in the blocking buffer and then incubated for 1 h at room temperature.

Samples were then added directly to ELISA plates, serially diluted in blocking buffer, and incubated at room temperature for 1 h.

The plates were blocked with casein blocking buffer and again incubated for one hour at 37°C on an orbital table.

After removing the blocking buffer from the plate, 20 μL of the preincubated competitor antibody mixture was applied to each well and the plate was incubated at room temperature for 2 h with gentle shaking.

After discarding blocking buffer from ELISA plates, duplicate 100-µl samples of 1∶100 diluted mouse serum were added to gD2306t -coated wells and were incubated for 2 hours.