Sentence examples for blocking buffer and placed from inspiring English sources

Heat-inactivated plasma were serially diluted from 1∶50 to 1∶1600 in blocking buffer and placed on the plates for 1 h at RT. Plates were washed three times with PBST.

Primary and secondary antibodies were diluted in the blocking buffer.

Antibodies and antiserum were diluted in blocking buffer and embryos incubated overnight in this solution at 4 °C.

Secondary antibodies were diluted in blocking buffer and applied to membranes for 1 h at room temperature.

Samples were then washed 3 × 30 minutes in blocking buffer and mounted on slides using Vectashield® (Vector Labs, Burlingame, CA).

Cells were subsequently washed three times in blocking buffer and incubated with the appropriate secondary antibody diluted in blocking buffer (donkey anti-rabbit AlexaFluor 647 or donkey anti-mouse AlexaFluor 647 (1:1000; Jackson ImmunoResearch)) for 2 hours at room temperature.

The cells were washed with the blocking buffer and passed through Cell Strainer (BD Biosciences, 40 µm pore size).

aureus antibody in blocking buffer, washed three times with blocking buffer, incubated for 20 minutes with 40 µg/ml Texas-RedX-conjugated goat anti-mouse IgG (Life Technologies) in blocking buffer, and washed twice with blocking buffer.

Cells were washed twice in blocking buffer and then incubated in 100 μl blocking buffer for 10 min with agitation.

Then, cells attached to the plate and from the supernatants were washed with PBS, resuspended in blocking buffer (BSA 1%-PBS) and placed on ice for 30 min. Cell surface staining of tumor cells was done with the following primary monoclonal antibodies: anti-Fas mAb (clone DX2) or anti-FasL mAb (clone NOK1) in blocking buffer for 1 h at 4°C.

Slides were placed in citrate buffer and microwaved for 20 minutes on low power, allowed to cool and placed in blocking buffer for 30 minutes, incubated with primary antibody overnight at 4°C, then washed and incubated with biotinylated secondary antibody (Vector Labs Universal horse anti-mouse/rabbit/goat #BA-1300 or Vector Labs Universal horse anti-mouse/rabbit #BA-1400) for 30 minutes.